Preservative Efficacy Test (10× Dilution)
- KIWITA
- Jan 15
- 2 min read
Test Report
No.: 13-102277
Date: October 9, 2013
1. Test Substance
Received September 5, 2013 – 1 sample
UMO Concentrated Solution (10× dilution)
Note: Prepared by our company by diluting with sterilized purified water 10-fold.
2. Test Method (According to JPXⅥ)
(1) Test Microorganisms
Escherichia coli NBRC 3972 (E. coli)
Pseudomonas aeruginosa NBRC 13275 (P. aeruginosa)
Staphylococcus aureus NBRC 13276 (S. aureus)
Candida albicans NBRC 1594 (C. albicans)
Aspergillus brasiliensis NBRC 9455 (A. brasiliensis)
(2) Preparation of Test Microbial Suspensions
Bacteria (E. coli, P. aeruginosa, S. aureus):Pre-cultured on SCD agar at 30°C for 20 hours. Cells were collected with a platinum loop, suspended in sterilized physiological saline, and adjusted to approximately 10⁸ cells/mL as the test suspension.
Yeast (C. albicans):Pre-cultured on potato dextrose agar at 25°C for 48 hours. Cells were collected with a platinum loop, suspended in sterilized physiological saline, and adjusted to approximately 10⁸ cells/mL as the test suspension.
Mold (A. brasiliensis):Pre-cultured on potato dextrose agar at 25°C for 7 days. Cells were collected with a platinum loop, suspended in 0.05% polysorbate 80 sterilized physiological saline, and adjusted to approximately 10⁷ cells/mL as the test suspension.
(3) Inoculation and StorageFor each test microorganism, 20 g of the test substance was placed in a sterilized vial, and 1% (0.2 mL) of the test microbial suspension was inoculated. Samples were stored at 25°C, and viable counts were measured on days 7, 14, 21, and 28.
(4) Measurement of Viable Cells
Bacteria: SCDLP agar plate count method
Fungi (yeast and mold): GPLP agar plate count method
3. Test Results
Table 1 – Viable Cell Counts at Receipt of Sample
(Original data table not included; likely shows initial counts of each microorganism upon receipt.)
Table 2 – Time Course of Viable Cell Counts After Inoculation
(Original data table not included; likely shows decrease in counts over 7, 14, 21, 28 days.)
4. Summary
The test substance showed good preservative efficacy against E. coli, P. aeruginosa, and S. aureus.
For C. albicans and A. brasiliensis, a clear reduction in inoculated cell numbers was observed, indicating that the preservative efficacy against fungi was also judged to be generally good.
Note: This paper is translated from the following URL. The content is provided for reference on the scientific research of the raw material only. Whether APA raw materials are used or not, we hope this research will help increase understanding and awareness of body minerals.
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