Measurement of Transepidermal Water Loss (TEWL) Before and After Application

KIWITA
Jan 15
2 min read

Updated: Jan 18

Measurement of Transepidermal Water Loss (TEWL)

Before and After Application

Test Number: CSA190207

Summary

The effect of UMO Concentrated Solution on transepidermal water loss (TEWL) was evaluated to verify its immediate effect on skin barrier function.

The TEWL at the site where the test product was applied was significantly lower than the control site 1 hour after application.

These results suggest that UMO Concentrated Solution has an immediate beneficial effect on the skin.

Test Method

The measurement site was washed with soap to remove sweat and dirt.
The subject remained at rest for 20 minutes in a constant temperature and humidity room (temperature: 21.0 ± 0.2°C, relative humidity: 45.0 ± 1.5%) to acclimate the skin to the test environment.
The test product was applied at a dosage of 5 μL/cm² to the measurement site.
Skin measurements were performed before application, 30 minutes after application, 1 hour after application, and 2 hours after application.
A no-application site was used as the operational control.

Measurement Instrument:

Transepidermal water loss (TEWL): Tewameter TM300, Courage + Khazaka, Germany¹⁻³

Results

The relative changes in TEWL were calculated using the pre-application TEWL as 100%.

Table 1 and Figure 1 show the relative TEWL changes,
Table 2 shows the individual relative values,
Table 3 shows the raw TEWL measurement values.

Interpretation:

Lower values indicate less water loss from the skin, suggesting improved barrier function.

References

G. E. Nillson, Med. Biol. Eng. Comput., 15(3), 209–218, 1977.
A. O. Barel and P. Clarys, Skin Pharmacol., 8(4), 186–195, 1995.
K. De Paepe, et al., Skin Res. Technol., 11(1), 61–69, 2005.

Table 1: Relative TEWL changes (%), pre-application = 100%

Figure 1: Relative TEWL changes (%), pre-application = 100%*n = 6, mean ± s.d., paired t-test, †P < 0.1, P < 0.05, *P < 0.01, **P < 0.001s.d.: standard deviation

Table 2: Individual relative TEWL values (pre-application = 100%)

Table 3: Individual TEWL measurement values

Note: This paper is translated from the following URL. The content is provided for reference on the scientific research of the raw material only. Whether APA raw materials are used or not, we hope this research will help increase understanding and awareness of body minerals.