Acute Oral Toxicity Test (Plant UMO Concentrated Solution)
- KIWITA
- Jan 15
- 3 min read
Acute Oral Toxicity Test
(Plant UMO Concentrated Solution)
February 28, 2019
Test Report
Client:
APA Corporation
Test Substance:
UMO Concentrated Solution (Husk Extract)
Title:
Acute Oral Toxicity Test Using Female Mice
We report the results of the test conducted on the above-mentioned sample, submitted to our center on January 22, 2019.
JFRL
No. 19007555001-0101, page 2/5
Acute Oral Toxicity Test Using Female Mice
Summary
An acute oral toxicity test (limit test) using female mice was conducted with UMO concentrated solution (husk extract) as the test substance. A single oral dose of 2000 mg/kg of the test substance was administered to female mice, and they were observed for 14 days. During the observation period, no abnormalities or deaths were observed.
From these results, it was concluded that the LD₅₀ value of the test substance in a single oral administration using female mice exceeds 2000 mg/kg.
1. Client
APA Corporation
2. Test Substance
UMO Concentrated Solution (Husk Extract)
3. Testing Facility
Tama Research Laboratory, Japan Food Research Laboratories6-11-10 Nagayama, Tama City, Tokyo
4. Test Period
January 22, 2019 – February 28, 2019
5. Test Objective
To investigate acute oral toxicity of the test substance in female mice in accordance with OECD Guideline for Testing of Chemicals 420 (2001).
6. Preparation of Test Solution
The test substance was diluted with injection water to prepare a 100 mg/mL test solution.
7. Test Animals
Five-week-old female ICR mice were purchased from Japan SLC Co., Ltd. After approximately one week of acclimatization and confirmation of normal general condition, the animals were used for the test. The mice were housed five per polycarbonate cage in a room at 23°C ± 3°C with a 12-hour light/dark cycle. Standard rodent diet (Lab MR Stock, Nihon Nosan Kogyo Co., Ltd.) and tap water were provided ad libitum.
8. Test Method
A test group receiving 2000 mg/kg of the test substance and a control group receiving injection water as a solvent control were established, with five mice per group. Animals were fasted for approximately 4 hours before dosing. After measuring body weight, the test group received the test solution, and the control group received injection water, at a dose of 20 mL/kg via oral gavage. Observations were made frequently on the day of dosing and once daily thereafter for 14 days. Body weight was measured on days 7 and 14 after dosing. Levene’s test was used to assess variance; since no significant difference was found, comparisons between groups were conducted using Student’s t-test, with a significance level of 5%. At the end of the observation period, all animals were necropsied.
9. Test Results
MortalityNo deaths occurred in any group during the observation period.
General ConditionNo abnormalities were observed in any group during the observation period.
Body Weight Changes (Table 1)Body weight measurements on days 7 and 14 after dosing showed no differences between the test and control groups.
Necropsy FindingsAt the end of the observation period, necropsy revealed no abnormalities in any of the animals.
10. Conclusion
An acute oral toxicity test (limit test) using female mice was conducted for the test substance. During the observation period, no abnormalities or deaths were observed. Therefore, the LD₅₀ of the test substance in female mice by single oral administration is considered to exceed 2000 mg/kg.
Administration Group Before Administration After administration (days)
7 Days. 14Days.
Test Group 26.9±1.0(5) 29.4±1.3(5) 31.2±0.8(5)
Control Group 26.5±0.8(5) 30.2±0.9(5) 31.5±0.5(5)
Table 1 – Body Weight Changes
Body weights are presented as mean ± standard deviation (unit: g). The number of animals is indicated in parentheses.
Note: This paper is translated from the following URL. The content is provided for reference on the scientific research of the raw material only. Whether APA raw materials are used or not, we hope this research will help increase understanding and awareness of body minerals.
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